Guanine nucleotide activation of adenylate cyclase in saponin permeabilized glioma cells
- 27 October 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 207 (2) , 296-301
- https://doi.org/10.1016/0014-5793(86)81508-3
Abstract
We have compared the regulation of adenylate cyclase activity in membrane fractions from C6 glioma cells and in monolayer cultures of C6 cells that had been permeabilized with saponin. Guanine nucleotides (GTP and GTPγS) and isoproterenol increase adenylate cyclase activity in C6 membranes and in permeabilized C6 cells. In C6 membranes, guanine nucleotides activate adenylate cyclase in the presence or absence of isoproterenol; in permeabilized cells, however, guanine nucleotides increase adenylate cyclase activity only in the presence of isoproterenol. We suggest that the properties of the permeabilized cells more closely resemble those of intact cells, and that some component which is present in permeabilized cells but is lost following cell disruption may be important for the normal regulation of adenylate cyclase activity.Keywords
This publication has 16 references indexed in Scilit:
- Exchange of guanine nucleotide between GTP-binding proteins that regulate neuronal adenylate cyclase.Proceedings of the National Academy of Sciences, 1986
- Catecholamine-stimulated GTPase cycle. Multiple sites of regulation by beta-adrenergic receptor and Mg2+ studied in reconstituted receptor-Gs vesicles.Journal of Biological Chemistry, 1986
- Two roles for guanine nucleotides in the stimulus-secretion sequence of neutrophilsNature, 1986
- Protein kinase C interferes with Ni‐mediated inhibition of human platelet adenylate cyclaseFEBS Letters, 1985
- Desipramine Elicits the Expression of Opiate Receptors and Sulfogalactosylceramide Synthesis in Rat C6Glioma CellsJournal of Neurochemistry, 1984
- Intracellular injection of guanyl nucleotides alters the serotonin-induced increase in potassium conductance in Aplysia neuron R15.The Journal of general physiology, 1984
- Role of Cytoskeletal Organization in the Regulation of Adenylate Cyclase-Cyclic Adenosine Monophosphate by HormonesEndocrine Reviews, 1983
- Alamethicin or detergent permeabilization of the cell membrane as a tool for adenylate cyclase determinationBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1982
- The regulatory subunit of adenylate cyclase interacts with cytoskeletal componentsNature, 1981
- Mechanism of adenylate cyclase activation through the beta-adrenergic receptor: catecholamine-induced displacement of bound GDP by GTP.Proceedings of the National Academy of Sciences, 1978