Kinetics of Conformational Change of Troponin C Induced by Calcium1
- 1 March 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 79 (3) , 689-691
- https://doi.org/10.1093/oxfordjournals.jbchem.a131114
Abstract
The kinetics of conformational change of troponin C (TN-C) induced by binding and removal of calcium ions were studied by measuring the fluorescence of tyrosine by stopped-flow spectrofluorometry. When the concentration of free calcium ions in the solution (Ca2+) was increased rapidly from 4×10−9M to 1×10−3M at neutral pH, a first-order reaction with a rate constant of 13.7 sec−1, which was preceded by a much faster reaction, was observed. In contrast, when (Ca2+) was reduced from 2×10−3M to 4.4×10−3M, two first-order reactions with rate constants of 7.4 and 0.78 sec−1, preceded by a much faster reaction, were observed.This publication has 0 references indexed in Scilit: