Glycoprotein Synthesis in Sertoli Cells During the Cycle of the Seminiferous Epithelium of Adult Rats: A Radioautographic Study

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Abstract
Glycoprotein synthesis in Sertoli cells was investigated in rats sacrificed at various intervals (10, 30 min; 1, 4, 8, 24 h) after a single intratesticular injection of [3H]fucose. Thin sections of glutaraldehyde-fixed testes were radioautographed and the relative concentration of the label over the various cytoplasmic components of the Sertoli cells was analyzed quantitatively from EM photographs. The analysis was performed at the various stages of the cycle of the seminiferous epithelium grouped as follows: I-IV, V-VII, VIII-XI, XII-XIV. The incorporation of [3H]fucose into glycoproteins took place initially in the Golgi apparatus of the cells and the labeled glycoproteins later migrated to the lysosomes and plasma membrane. The corrected grain counts over the various cytoplasmic components of the Sertoli cell at various intervals after [3H]fucose injection indicated that the synthesis of glycoproteins detected by radioautography after [3H]fucose injection was constant during the cycle of the seminiferous epithelium. The rate of transfer of the labeled glycoproteins from the Golgi apparatus to the lysosomes and plasma membrane was also noncyclic.

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