Specific techniques for the identification of O‐acylated sialic acids in colonic mucins
- 2 August 1980
- journal article
- Published by Wiley in Journal of Microscopy
- Vol. 119 (3) , 415-425
- https://doi.org/10.1111/j.1365-2818.1980.tb04113.x
Abstract
The development of methodology for the histochemistry of mucins based upon their PAS reactivity is discussed in terms of mechanism, specificity and application. Two new histochemical methods (PB/KOH/PAS and PAT/KOH/PAS), supplemented by a variety of new and standard histochemical techniques, and correlated by parallel chemical studies, were used to demonstrate and identify C4 and side chain O-acylated sialic acids in colonic epithelial mucins. The application of these methods in the field of histopathology is discussed.Keywords
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