The activation and inhibition of 5-nucleotidase

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Abstract
5-Nucleotidase is activated strongly by Mn++ ions. Nickel (Ni++) and Zn++ ions inhibit 5-nucleotidase activity. Ni++ in millimolar concentration almost completely inhibits the 5-nucleotidase, whereas nonspecific phosphatase activity is not affected. The possibility of using nickel inhibition for 5-nucleotidase estimations is discussed. Phosphate inhibits 5-nucleotidase to a much smaller extent than it does alkaline phosphatase.
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