AN EUGLYCEMIC HYPERINSULINIC GLUCOSE CLAMP TECHNIQUE - ITS FEASIBILITY AND REPRODUCTIBILITY

  • 1 January 1984
    • journal article
    • research article
    • Vol. 10  (1) , 36-38
Abstract
The euglycemic hyperinsulinemic clamp is a means for the study of insulin sensitivity. Several techniques have been described: some are manual, others are automatic and use hypertonic glucose (50%). A semi-automatic technique is described using an artificial .beta. cell and a pump. The method and its reproducibility in 10 healthy subjects were studied. The choice of appropriate algorithms insures a constant and stable insulin level of 93.3 .+-. 5 M U/ml and good glycemic feedback through graduated glucose perfusion. The use of a supplementary pump in series with an artificial pancreas insures basic glucose input without recourse to strong concentrations (20%); glycemic feedback of the artificial pancreas is thereby dynamically controlled. The glucose requirement is 8.08 .+-. 0.73 mg/kg per min. The same values are found in the same patients 3 wk later and resemble those obtained using other techniques. Automatic feedback makes the technique objective; low concentration glucose solution avoids damage to veins.

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