Formation, Fusion, and Regeneration of Protoplasts from Wild-Type and Auxotrophic Strains of the White Rot Basidiomycete Phanerochaete chrysosporium
- 1 July 1983
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 46 (1) , 260-263
- https://doi.org/10.1128/aem.46.1.260-263.1983
Abstract
A preparation of two commercial enzymes was used to liberate protoplasts from 16-h-old mycelium of Phanerochaete chrysosporium. Regeneration frequencies of up to 5% were attained when the protoplasts were plated in a medium containing 10% sorbose and 3% agar. Fusion of protoplasts from different auxotrophic strains in polyethylene glycol-Ca 2+ produced heterokaryons. Separation of the heterokaryons into their constituent homokaryotic strains could be effected through protoplast release and formation of colonies on regeneration agar.This publication has 7 references indexed in Scilit:
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