RADIOIMMUNOASSAYS OF TETRAHYDROCORTISONE AND TETRAHYDROCORTISOL IN HUMAN URINE

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Abstract
A simple radioimmunoassay for the determination of tetrahydrocortisone (THE), and tetrahydrocortisol (THF) as well as their 21-glucosiduronate conjugates is described. White New Zealand rabbits were immunized with THF- and THE-20-oxime-bovine serum-albumin conjugates. The resulting antisera were very specific. Both allo-THE and allo-THF gave minimal cross-reactions; however, THE- and THF-21-glucosiduronate bind with the corresponding antibodies at a level of 100%. Estimation was made of the sum of unconjugated THE and THF-21-glucosiduronate in highly diluted unprocessed human urine. A similar estimation of THE and THF-21-glucosiduronate was made. Estimation was made of THF after .beta.-glucuronidase treatment and dilution without further procedures. A similar estimation for THE was made. THF and THE values correlate significantly with known parameters that characterize the cortisol production, such as cortisol secretion rate and the urinary corticoid (C21-.alpha.-ketolic-steroid) levels.

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