Insulin and Insulin-Like Growth Factor-I(IGF-I) Stimulate the Development of Bovine Embryos Fertilized In Vitro.
- 1 January 1995
- journal article
- research article
- Published by Japanese Society of Veterinary Science in The Journal of Veterinary Medical Science
- Vol. 57 (6) , 1109-1111
- https://doi.org/10.1292/jvms.57.1109
Abstract
To study the effects of insulin and insulin-like growth factor-I (IGF-I) on early development of bovine embryos, one-cell embryos were cultured in a chemically defined, protein-free medium: modified synthetic oviduct fluid. The addition of 10 μg/ml insulin or 20 ng/ml IGF-I increased the percentage of morulae at Day 5 (5 days after insemination). The cell number of blastocysts which developed after 2 days culture of morulae obtained at Day 5 was increased by supplementation with insulin or IGF-I. The combination of insulin and IGF-I had neither additive nor synergistic effect on the embryonic development. These results clearly show that insulin and IGF-I improve the in vitro development of bovine embryos.Keywords
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