Effect of surface-active agent on the active transport of sodium in frog skin.

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Abstract
The effect of surface-active agent was studied on the active transport of Na in the frog skin. Alkyldimethylbenzylammonium chloride (alkyl-DBAC) in 10-3 M concentration inhibited the active transport of Na accompanied by Na+-K+ ATPase, whereas it increased the influx and outflux of 24Na through the frog skin probably due to structural damage of the frog skin. This effect was observed above the critical micellar concentration of this surfactant. The increased influx of 24Na was decreased by the addition of 1 .mu.g Pi/ml of phospholipid to 10-3 M alkyl-DBAC, but it still remained at a higher level. The effect of phospholipase C on the active transport of Na in the frog skin was different from that of alkyl-DBAC, and 0.5 mg/ml of phospholipase C also increased the influx of 24Na, whereas the increased influx of 24Na was depressed by 10-5 M ouabain.