Abstract
Neural crest cells obtained from Japanese quail and grown in vitro without other embryonic tissues differentiated into adrenergic cells. These cells showed intense catecholamine-specific histochemical fluorescence and some had long, varicose neuronal processes. Ultrastructural examination showed 2 populations of cells, 1 with small (about 90 nm) dense-core vesicles resembling principal sympathetic neurons and the other with larger (about 150 nm) dense-core granules resembling chromaffin or small intensely fluorescent cells. Neuronal cells without adrenergic characteristics were also present. A population of cells determined along neuronal lines existed in the neural crest prior to migration.

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