The Use of Monoclonal Antibodies to Define Levels of Cystatin C in Normal Human Serum
- 1 June 1989
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 8 (3) , 303-313
- https://doi.org/10.1089/hyb.1989.8.303
Abstract
We established four hybridoma cell lines which secreted monoclonal antibodies against human cystatin C. These monoclonal antibodies were of IgA(κ), IgG2a(λ), IgG1(κ), and IgG1(λ) isotypes, respectively. The association constants (Ka) of the three IgG monoclonal antibodies ranged from 3.6 x 109 M-1 to 7.3 x 1010 M-1. An ELISA technique for the measurement of cystatin C was established by using one of these monoclonal antibodies. The assay procedure was highly specific, sensitive, and reproducible. The lower detectable limit of cystatin C by this procedure was 1.9 ng/ml. The level of cystatin C in normal human serum was 1.16 ± 0.91 μg/ml (mean ± S.D., n=274).This publication has 28 references indexed in Scilit:
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