Submucosal microinfusion of endothelin and adrenaline mobilizes ECL‐cell histamine in rat stomach, and causes mucosal damage: a microdialysis study

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Abstract
Rat stomach ECL cells release histamine in response to gastrin. Submucosal microinfusion of endothelin or adrenaline, known to cause vasoconstriction and gastric lesions, mobilized striking amounts of histamine. While the histamine response to gastrin is sustainable for hours, that to endothelin and adrenaline was characteristically short‐lasting (1–2 h). The aims of this study were to identify the cellular source of histamine mobilized by endothelin and adrenaline, and examine the differences between the histamine‐mobilizing effects of gastrin, and of endothelin and adrenaline. Endothelin, adrenaline or gastrin were administered by submucosal microinfusion. Gastric histamine mobilization was monitored by microdialysis. Local pretreatment with the H1‐receptor antagonist mepyramine and the H2‐receptor antagonist ranitidine did not prevent endothelin‐ or adrenaline‐induced mucosal damage. Submucosal microinfusion of histamine did not cause damage. Acid blockade by ranitidine or omeprazole prevented the damage, suggesting that acid back diffusion contributes. Gastrin raised histidine decarboxylase (HDC) activity close to the probe, without affecting the histamine concentration. Endothelin and adrenaline lowered histamine by 50–70%, without activating HDC. Histamine mobilization declined upon repeated administration. Endothelin reduced the number of histamine‐immunoreactive ECL cells locally, and reduced the number of secretory vesicles. Thus, unlike gastrin, endothelin (and adrenaline) is capable of exhausting ECL‐cell histamine. Microinfusion of α‐fluoromethylhistidine (known to deplete ECL cells but not mast cells of histamine) reduced the histamine‐mobilizing effect of endothelin by 80%, while 1‐week pretreatment with omeprazole enhanced it, supporting the involvement of ECL cells. Somatostatin or the prostanoid misoprostol inhibited gastrin‐, but not endothelin‐stimulated histamine release, suggesting that endothelin and gastrin mobilize histamine via different mechanisms. While gastrin effectively mobilized histamine from ECL cells in primary culture, endothelin had no effect, and adrenaline, a modest effect. Hence, the striking effects of endothelin and adrenaline on ECL cells in situ are probably indirect, possibly a consequence of ischemia. British Journal of Pharmacology (2003) 140, 707–717. doi:10.1038/sj.bjp.0705473

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