Isolation and Mapping of Glutathione Reductase-negative Mutants of Escherichia coli K12
- 1 July 1982
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 128 (7) , 1631-1634
- https://doi.org/10.1099/00221287-128-7-1631
Abstract
Two independent mutants defective in glutathione reductase (EC 1.6.4.2) were isolated in an E. coli K12 strain lysogenized with phage Mu. The prophage was lost (and the ability to reduce glutathione regained) by 32% of the xylose-positive transductants when T4GT7 was used as the vector, but the markers were not cotransduced by phage P1. Similarly, the prophage site and malA were cotransduced by T4GT7 but not by P1. The gor gene maps between min 77 and 78 on the E. coli genome, and the mutation causes no growth defect.Keywords
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