Studies of Biologic and Serologic Activities of Rabbit-IgG Antibody Depleted of Carbohydrate Residues
Open Access
- 1 December 1973
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 111 (6) , 1690-1698
- https://doi.org/10.4049/jimmunol.111.6.1690
Abstract
Rabbit IgG preparations containing antibody activity against individual strains of Staphylococcus aureus, Escherichia coli, group A β-hemolytic streptococci, and pneumococci were digested with Diplococcus pneumoniae endoglycosidase enzyme with removal of more than half of Ig-associated carbohydrate. In addition specifically purified rabbit anti-bovine serum albumin (BSA) IgG was similarly treated. After carbohydrate removal no appreciable change in opsonic, complement fixing, or agglutinating activity was noted in anti-staphylococcal, anti-E. coli, or anti-streptococcal IgG preparations, whereas complete loss of both complement fixing and opsonic activity occurred with rabbit IgG containing anti-pneumococcal antibody activity. No change in passive cutaneous anaphylactic reactions was noted after carbohydrate depletion of immunoabsorbent isolated rabbit anti-BSA IgG antibody. These findings indicate heterogeneous influences of carbohydrate residues on biologic activities of various antibody populations.Keywords
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