Membrane transport ofp-nitrophenyl-α-galactoside by the melibiose carrier ofEscherichia coli
- 1 June 1980
- journal article
- research article
- Published by Springer Nature in The Journal of Membrane Biology
- Vol. 56 (2) , 169-175
- https://doi.org/10.1007/bf01875968
Abstract
Summary p-Nitrophenyl-α-galactoside (α-pNPG) was found to be a substrate for the melibiose transport system ofEscherichia coli. This sugar enters induced cells via the carrier and is split by α-galactosidase to galactose andp-nitrophenol. In mutant cells lacking the α-galactosidase [3H]-α-pNPG accumulated to concentrations 15 times higher than the external medium. The transport of α-pNPG is inhibited by both Na+ and Li+. Na+ (10mm) reduced the Km for α-pNPG from 0.45 to 0.18mm and reduced theV max from 6.7 nmoles/min/mg dry wt to a value of 3.0.Keywords
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