INHIBITION OF PHYTOHEMAGGLUTININ-INDUCED PROLIFERATION OF HUMAN PERIPHERAL-BLOOD LYMPHOCYTES BY HISTAMINE AND HISTAMINE H-1 AND H-2 AGONISTS

  • 1 January 1983
    • journal article
    • research article
    • Vol. 51  (1) , 178-184
Abstract
Histamine (10-3 to 10-8 M) inhibited PHA[phytohemagglutinin]-induced proliferation of human peripheral blood lymphocytes (HPBL). Inhibition was detected at low concentrations of PHA but was rarely observed at high PHA concentrations. The histamine type II (H2) receptor agonists dimaprit, impromidine and 4-methylhistamine (4MH) inhibited HPBL proliferation, and the H2 antagonist, cimetidine, reversed histamine-induced suppression of HPBL proliferation. Lymphocyte proliferation was inhibited by the H1 receptor agonists, 2-pyridylethylamine and 2-thiazoylylethylamine, but only at high concentrations (10-3 and 10-4 M). The H1 agonist 2-methylhistamine suppressed PHA-induced proliferation of HPBL in analogous fashion to histamine. This effect was reversed by cimetidine, but not by diphenhydramine, suggesting that an H2 receptor interaction was involved.