Development of -Type Energy Filtering TEM

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Abstract
A new energy-filter is developed to improve image resolution and enhance the contrast of transmission electron microscopic (TEM) images. The filter consists of two sector magnetic fields and is built into a TEM column. Electrons travel through the filter following γ-shape trajectories. Electrons in a specific range of energies are selected and focused to produce an image. The energy filter was incorporated into a TEM for observation of unstained biological specimens. With a 1-μm thick specimen, zero-loss electrons produce a sharper, or less chromatic-aberration image compared with that of a conventional TEM. Meanwhile, with a thin specimen less than 0.1 μm thick, around 250eV-loss electrons produce a sharp image while zero-loss electrons do not. Observations show that a TEM equipped with the energy filter is useful for microscopy of fine structures of biological specimens under natural conditions.

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