Autoradiographic Evidence of [3H]SCH 23390 Binding Site; in Human Prefrontal Cortex (Brodmann's Area 9)
- 1 September 1987
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 49 (3) , 789-796
- https://doi.org/10.1111/j.1471-4159.1987.tb00962.x
Abstract
The distribution of dopamine D-1 receptors has been determined in human prefrontal cortex (Brodmann''s area 9) by an in vitro light microscopic autoradiographic method. Dopamine D-1 receptors are localized by using [3H]SCH 23390 as a ligand. Our results indicated that [3H]-SCH 23390 binding to slide-mounted tissue sections of human brain is specific, saturable, and of high affinity. Lamina Va contained the highest density of D-1 receptors, with a Bmax value of 11.2 .+-. 1.3 fmol/mg tissue. The KD values for [3H]SCH 23390 in all laminae ranged from 2.6 to 3.2 .mu.M. Competition studies performed with [3H]SCH 23390 indicated a pharmacologic profile consistent with labeling of the D-1 receptor.Keywords
This publication has 38 references indexed in Scilit:
- Quantitative autoradiographic localization of D-1 dopamine receptors in the rat brain: Use of the iodinated ligand [125I]SCH 23982Neuroscience Letters, 1986
- D-2 dopamine receptors in the frontal cortex of rat and humanLife Sciences, 1985
- Characterization of the binding of 3H-SCH 23390, a selective D-1 receptor antagonist ligand, in rat striatumLife Sciences, 1984
- Regional and Cortical Laminar Distributions of Serotonin S2, Benzodiazepine, Muscarinic, and Dopamine D2 Receptors in Human BrainJournal of Neurochemistry, 1984
- Effects of Postmortem Delay and Temperature on Neurotransmitter Receptor Binding in a Rat Model of the Human Autopsy ProcessJournal of Neurochemistry, 1984
- Human brain neurochemistry — Some postmortem problemsLife Sciences, 1983
- [3H]Dopamine Labeling of D3Dopaminergic Sites in Human, Rat, and Calf BrainJournal of Neurochemistry, 1982
- Quantitative measurement of local cerebral metabolic rate for glucose utilizing tritiated 2-deoxyglucoseBrain Research, 1981
- Catecholamines in neocortex of rhesus monkeys: regional distribution and ontogenetic developmentBrain Research, 1977
- Dopaminergic Terminals in the Rat CortexScience, 1973