Kinetics of Na+-dependent amino acid transport using cells and membrane vesicles of a marine pseudomonad

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Abstract
Sodium ion is required for transport of L-alanine and α-aminoisobutyric acid (AIB) into cells and membrane vesicles of a marine pseudomonad. Initial rate data obtained at various Na+ and amino acid concentrations were tested for fit by least squares analysis to sequential and Ping-Pong equations. The sequential case is preferred statistically at the 99% confidence limit. Cotransport of AIB and Na+ in cells could not be detected, perhaps explained by efflux of Na+ shown to occur from these cells. The kinetic analysis is consistent with formation of a ternary complex involving Na+ and the amino acid.