Genetic Analysis of Radiation-sensitive Mutations in the Slime Mould Dictyostelium discoideum

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Abstract
The linkage of 2 mutations leading to increased sensitivity to UV light and 60Co .gamma.-rays was determined in the slime mold. D. discoideum using a genetic analysis based on the parasexual cycle. Diploids were selected from a mixture of radiation-sensitive, temperature-resistant and radiation-resistant, temperature-sensitive haploids on the basis of simultaneous radiation and temperature resistance. Analysis of drug-resistant haploid segregants of the heterozygous diploids indicated that one of the radiation-sensitive mutations, radA20, was linked to linkage group I whereas the other, radB13, was linked to the recently defined linkage group VI.