Cloned Ca2+-dependent K+ channel modulated by a functionally associated protein kinase
- 1 June 1994
- journal article
- Published by Springer Nature in Nature
- Vol. 369 (6481) , 563-565
- https://doi.org/10.1038/369563a0
Abstract
Calcium-dependent potassium (KCa) channels carry ionic currents that regulate important cellular functions. Like some other ion channels, KCa channels are modulated by protein phosphorylation. The recent cloning of complementary DNAs encoding Slo KCa channels has enabled KCa channel modulation to be investigated. We report here that protein phosphorylation modulates the activity of Drosophila Slo KCa channels expressed in Xenopus oocytes. Application of ATP-gamma S to detached membrane patches increases Slo channel activity by shifting channel voltage sensitivity. This modulation is blocked by a specific inhibitor of cyclic AMP-dependent protein kinase (PKA). Mutation of a single serine residue in the channel protein also blocks modulation by ATP-gamma S, demonstrating that phosphorylation of the Slo channel protein itself modulates channel activity. The results also indicate that KCa channels in oocyte membrane patches can be modulated by an endogenous PKA-like protein kinase which remains functionally associated with the channels in the detached patch.Keywords
This publication has 27 references indexed in Scilit:
- Phosphorylation of amino acid neurotransmitter receptors in synaptic plasticityTrends in Neurosciences, 1993
- An Amino Acid Mutation in a Potassium Channel That Prevents Inhibition by Protein Kinase CScience, 1992
- Modulation by cAMP of a slowly activating potassium channel expressed in Xenopus oocytesJournal of Neuroscience, 1992
- Functional Modulation of Brain Sodium Channels by Protein Kinase C PhosphorylationScience, 1991
- Phosphorylation of the R domain by cAMP-dependent protein kinase regulates the CFTR chloride channelCell, 1991
- Calcium activates two types of potassium channels in rat hippocampal neurons in cultureJournal of Neuroscience, 1991
- Identification of phosphorylation sites for adenosine 3',5'-cyclic phosphate dependent protein kinase on the voltage-sensitive sodium channel from Electrophorus electricusBiochemistry, 1989
- Vasoactive intestinal peptide activates Ca2+ -dependent K+ channels through a cAMP pathway in mouse lacrimal cellsNeuron, 1988
- Purified dihydropyridine-binding site from skeletal muscle t-tubules is a functional calcium channelNature, 1986
- Two distinct Ca-dependent K currents in bullfrog sympathetic ganglion cells.Proceedings of the National Academy of Sciences, 1985