RELATIONSHIP BETWEEN INCORPORATION OF 9-BETA-D-ARABINOFURANOSYLADENINE IN L1210 DNA AND CYTO-TOXICITY

  • 1 January 1983
    • journal article
    • research article
    • Vol. 43  (5) , 2000-2004
Abstract
Cesium sulfate density gradient centrifugation was used to monitor the incorporation of 9-.beta.-D-arabinofuranosyladenine (ara-A) into L1210 [mouse leukemia cell line] cellular nucleic acids. The results demonstrate the specific incorporation of ara-A in L1210 DNA. A highly significant relationship was found between the formation of ara-A incorporated into DNA and loss of clonogenic survival. This relationship was maintained when using ara-A in the presence of the adenosine deaminase inhibitor deoxycoformycin. Treatment with increasing concentrations of ara-A resulted in a greater proportion of ara-A residues at the 3''-terminus, consistent with this agent providing a poor primer terminus for elongating DNA strands. These findings are similar to those obtained previously with 1-.beta.-D-arabinofuranosylcytosine and suggest that the incorporation of arabinofuranosyl derivatives in DNA is one mechanism responsible for cell lethality.

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