Technical Procedures for the Study of the Nucleolonema

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Abstract
In a recent paper1 the authors have shown filamentous structures within the nucleolus of all cells. This structure was named the nucleolonema. In the present paper the technical procedures which prove their existence are described in detail. There are two groups: a) Silver impregnation procedures and iron-pyrogallic stain for fixed material; b) phase contrast microscopy, dark-field illumination and oblique transillumination for fresh material. The suitable objects are mentioned in every case in order to obtain the best results.

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