Differential Helix Stabilities and Sites Pre-organized for Tertiary Interactions Revealed by Monitoring Local Nucleotide Flexibility in the bI5 Group I Intron RNA
- 8 January 2003
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 42 (4) , 901-909
- https://doi.org/10.1021/bi026817h
Abstract
The local environment at adenosine residues in the bI5 group I intron RNA was monitored as a function of Mg2+ using both the traditional method of dimethyl sulfate (DMS) N1 methylation and a new approach, selective acylation of 2‘-amine substituted nucleotides. These probes yield complementary structural information because N1 methylation reports accessibility at the base pairing face, whereas 2‘-amine acylation scores overall residue flexibility. 2‘-Amine acylation robustly detects RNA secondary structure and is sensitive to higher order interactions not monitored by DMS. Disruption of RNA structure due to the 2‘-amine substitution is rare and can be compensated by stabilizing folding conditions. Peripheral helices that do not interact with other parts of the RNA are more stable than both base paired helices and tertiary interactions in the conserved catalytic core. The equilibrium state of the bI5 intron RNA, prior to assembly with its protein cofactor, thus features a relatively loosely packed core anchored by more stable external stem−loop structures. Adenosine residues in J4/5 and P9.0 form structures in which the nucleotide is constrained but the N1 position is accessible, consistent with pre-organization to form long-range interactions with the 5‘ and 3‘ splice sites.Keywords
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