FACTORS AFFECTING THE GROWTH OF ENTEROCOCCI IN HIGHLY ALKALINE MEDIA

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Abstract
Growth of enterococci in media having a high initial pH is stimulated markedly by carbonate and to a lesser extent by oleate. Growth in these media is inhibited by glycine and by the D-isomer of a variety of amino acids. Peptides of glycine are less inhibitory than the free acid. In a favorable medium, buffered with carbonate rather than glycine, all. enterococci tested initiated growth up to pH 10.5. Such a medium adjusted to pH 10.0 has been shown to be a superior enrichment broth for the detection of enterococci in fecal samples and presumably from other sources. It also provides a convenient taxonomic tool for differentiating enterococci from other streptococci.

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