Unusual promoter-independent transcription reactions with bacteriophage RNA polymerases
Open Access
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (8) , 3023-3036
- https://doi.org/10.1093/nar/17.8.3023
Abstract
Efficient transcription reactions of DNA-dependent RNA polymerases require the presence of a specific promoter sequence. This report shows that in the absence of their cognate promoter, two bacteriophage RNA polymerases are capable of performing unusual transcription reactions: (i) the DNA template serves also as a primer for RNA synthesis and this leads to hybrid DNA/RNA molecules, (ii) if the DNA template forms a hairpin structure, the linear DNA can be transcribed via the ‘rolling circle’ mechanism.This publication has 26 references indexed in Scilit:
- RNA synthesis: strategies for the use of bacteriophage RNA polymerasesGene, 1988
- In vitro synthesis of 16S ribosomal RNA containing single base changes and assembly into a functional 30S ribosomeBiochemistry, 1987
- Speculations on the early course of evolution.Proceedings of the National Academy of Sciences, 1986
- Sequences of three promoters for the bacteriophage SP6 RNA polymeraseNucleic Acids Research, 1986
- Transcription from bacteriophage T7 and SP6 RNA polymerase promoters in the presence of 3'-deoxyribonucleoside 5'-triphosphate chain terminatorsBiochemistry, 1985
- The role of RNA priming in viral and trypanosomal mRNA synthesisCell, 1985
- Structure and Transcription of Eukaryotic tRNA GeneCritical Reviews in Biochemistry, 1985
- Recognition of cap structure in splicing in vitro of mRNA precursorsCell, 1984
- The Amplification of Ribosomal RNA Genes Involves a Rolling Circle IntermediateProceedings of the National Academy of Sciences, 1973
- DNA Replication: The Rolling Circle ModelCold Spring Harbor Symposia on Quantitative Biology, 1968