Yellow Fever Virus Replicons as an Expression System for Hepatitis C Virus Structural Proteins
Open Access
- 15 January 2003
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 77 (2) , 1644-1648
- https://doi.org/10.1128/jvi.77.2.1644-1648.2003
Abstract
Chimeric yellow fever virus (YF) RNAs were constructed in which the YF structural genes were replaced by the hepatitis C virus (HCV) structural genes or fusions between the YF and HCV structural genes. Interestingly, RNA replication required nucleotide complementarity between the 3′-located conserved sequence 1 and an RNA sequence located in the 5′ end of the YF capsid sequence. The (chimeric-)HCV structural proteins were efficiently expressed and processed, and the native E1/E2 heterodimer was formed. However, no indication for the production of HCV-like particles was obtained.Keywords
This publication has 51 references indexed in Scilit:
- Mutations in the Yellow Fever Virus Nonstructural Protein NS2A Selectively Block Production of Infectious ParticlesJournal of Virology, 2002
- Hepatitis C Virus-Like Particle MorphogenesisJournal of Virology, 2002
- Persistent and Transient Replication of Full-Length Hepatitis C Virus Genomes in Cell CultureJournal of Virology, 2002
- Selectable Subgenomic and Genome-Length Dicistronic RNAs Derived from an Infectious Molecular Clone of the HCV-N Strain of Hepatitis C Virus Replicate Efficiently in Cultured Huh7 CellsJournal of Virology, 2002
- Construction, Safety, and Immunogenicity in Nonhuman Primates of a Chimeric Yellow Fever-Dengue Virus Tetravalent VaccineJournal of Virology, 2001
- Essential Role of Cyclization Sequences in Flavivirus RNA ReplicationJournal of Virology, 2001
- Secondary structure of the 3' untranslated region of flaviviruses: similarities and differencesNucleic Acids Research, 1997
- An infectious arterivirus cDNA clone: Identification of a replicase point mutation that abolishes discontinuous mRNA transcriptionProceedings of the National Academy of Sciences, 1997
- Conserved elements in the 3′ untranslated region of flavivirus RNAs and potential cyclization sequencesJournal of Molecular Biology, 1987
- Nucleotide Sequence of Yellow Fever Virus: Implications for Flavivirus Gene Expression and EvolutionScience, 1985