Modulation of phospholipase A2 activity in human fibroblasts

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Abstract
1 Human embryonic skin fibroblasts (HSF) incubated overnight with either human recombinant interleukin-1α (rIL-1α) or interleukin-1β (rIL-1β) released large amounts of prostaglandin E2 (PGE2). 2 rIL-1β, bradykinin (Bk) and arachidonic acid (AA) significantly stimulated PGE2 release from HSF incubated overnight in the presence of either interleukin. 3 Hydrocortisone inhibited the PGE2 release induced by rIL-1β and Bk, but not by AA. 4 The steroid inhibitory effect was reversed by actinomycin D as well as by an anti-lipocortin monoclonal antibody. 5 The results suggest that in HSF, rIL-1β is able to stimulate both cyclo-oxygenase and phospholipase A2 (PLA2) activity. 6 The stimulation of PLA2 activity by rIL-1β is inhibited by hydrocortisone, probably via induction of lipocortin-like proteins.