Inhibition Experiments with Solid Phase Mite or Epithelia in House Dust Hypersensitivity

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Abstract
The present study was undertaken to verify that mites are not the only allergens in house dust extracts and that other allergens such as cat epithelia can also be responsible for house dust hypersensitivities detected by house dust skin tests and house dust RAST [radioallergosorbent test] studies. To determine whether mite or epithelia fixed on a solid phase could remove the Ig[immunoglobulin]E antibodies reactive with the homologous allergens and the IgE antibodies reactive with house dust allergens, 10 sera of house dust allergic patients were absorbed with solid phase mite of epithelia. The absorption procedure removed a large part of the IgE antibodies reactive with specific immunosorbent (D. pteromyssinus or cat epithelia) and in the same way the IgE antibodies reactive with house dust immunosorbent. The percentage of RAST inhibition varied from 65-92% for D. pteromyssinus and from 65-94% for house dust in patients allergic to house dust and mite; the percentage of RAST inhibition varied from 67-92% for cat epithelia and from 73-90% for house dust in patients allergic to house dust and cat epithelia. This is in accordance with the hypothesis that house dust is not an allergen per se, but rather a complex mosaic of several allergens including mite, animal epithelia, etc.