Production ofl-Glutamic Acid fromdl-Hydantoin-5-propionic Acid by Microoganisms

Abstract
Screening tests for obtaining microorganisms which can produce L-glutamic acid from hydantoin-5-propionic acid were carried out. Many strains of microorganism were found to possess this ability. It was newly found that the glutamic acid produced by these strains was exclusively L-form regardless of the stereochemical configuration of the substrate used. Among the selected strains Bacillus brevis ATCC 8185 showed the highest ability to produce L-glutamic acid. Some investigations for obtaining optimal conditions for producing L-glutamic acid using washed cells of Bacillus brevis ATCC 8185 were carried out. Under the adequate conditions using the reaction mixture containing 1.0% of DL-hydantoin-5-propionic acid, washed cells of this strain converted 90% of the DL-acid to L-glutamic acid. An assumptive pathway of producing L-glutamic acid from D-hydantoin-5-propionic acid was also discussed.

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