PHOSPHORUS METABOLISM IN BACILLUS MEGATERIUM DURING SPORULATION

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Abstract
Phosphorus metabolism in sporu-lating B. megaterium was examined by the fractionation of P compounds in cells. Marked changes in the amount of various P fractions prior to and during the endospore formation suggested that the intracellular reconstruction was occurring in these phases of spore formation. The contribution of intracellular P reserves, especially of polyphosphate, to the sporulation process was suggested. Conspicuous decreases (up to 1/3) in the amount of RNA and DNA during the course of liberation of free spores was observed. This was discussed in relation to the mode of free spore liberation.