Phorbol ester induces loss of VIP stimulation of adenylate cyclase and VIP‐binding sites in HT29 cells
- 26 January 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 211 (2) , 151-154
- https://doi.org/10.1016/0014-5793(87)81426-6
Abstract
Treatment of HT29 cells with the tumor promoting phorbol ester PMA resulted in an attenuation of VIP-stimulated cAMP production in intact cells and VIP-stimulated adenylate cyclase activity in cell membranes. PMA did not decrease the ability of cholera toxin and forskolin to elevate cAMP levels in intact cells. Fluoride-stimulated adenylate cyclase activity in HT29 cells homogenates was not affected byPMA. The maximal VIP binding capacity of homogenates prepared from HT29 cells treated with PMA was decreased by 50%. It is concluded that protein kinase C regulates VIP receptor function possibly through phosphorylation of the VIP receptor.Keywords
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