EFFECTS OF HYPOTHALAMIC EXTRACT AND STEROIDS ON THE SECRETION OF PROLACTIN, GROWTH HORMONE AND LUTEINIZING HORMONE BY THE PITUITARY GLAND OF THE TERRAPIN (CHRYSEMYS PICTA) IN VITRO

Abstract
Pituitary glands were incubated with 25 .mu.g progesterone, 25 .mu.g testosterone, 25 ng estradiol-17.beta., an amount of hypothalamic extract (HE) equivalent to 1 hypothalamus or 1 of the steroids plus HE. All 3 steroids significantly (P < 0.05) stimulated the secretion of LH [luteinizing hormone], compared with results obtained from incubation in Medium 199 alone. In C. picta the secretion of prolactin and GH [growth hormone] in vitro is influenced by hypothalamic releasing factors. Progesterone, estradiol-17.beta. and testosterone also act alone to influence the secretion of pituitary hormones in vitro.

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