LIPOSOMES AS MODEL MEMBRANE SYSTEMS FOR IMMUNE ATTACK .1. TRANSFER OF ANTIGENIC DETERMINANTS TO LYMPHOCYTE MEMBRANES AFTER INTERACTIONS WITH HAPTEN-BEARING LIPOSOMES

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 121  (4) , 1376-1382
Abstract
The interaction of human peripheral blood lymphocytes with liposomes containing DNP[dinitrophenyl]-aminocapropylphosphatidylethanolamine with egg yolk or dipalmitoylphosphatidylcholine was investigated. When lymphocytes were incubated with liposomes at 37.degree. C, the aqueous compartment (86Rb+) and the lipid portion (3H-lipid) of the liposomes became cell associated to an equivalent extent. At 0.degree. C the incorporation of 3H-lipid exceeded that of 86Rb+. Lymphocyte-liposome interactions were accompanied by the transfer of DNP to the surface of the lymphoid cell as measured by susceptibility to complement in the presence of anti-DNP antibody. Hapten transfer was not limited to liposome interactions with lymphocytes, but occurred with other cells (e.g., Chang cells). Hapten transfer could be demonstrated by susceptibility to K [antibody dependent killer cells] cell-mediated lysis. Liposomes may be a useful vehicle for the transfer of new antigenic determinants onto cell surfaces. The possible use of liposomes as targets for cell-mediated cytotoxic attack was discussed.