Sequence and identification of the nucleotide binding site for the elongation factor Tu fromThermus thermophilusHB8
Open Access
- 25 November 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (22) , 9263-9277
- https://doi.org/10.1093/nar/15.22.9263
Abstract
Two structural genes for the Thermus thermophilus elongation factor Tu (tuf) were identified by cross-hybridization with the tufA gene from E.coli. The sequence of one of these tuf genes, localized on a 6.6 kb Bam HI fragment, was determined and confirmed by partial protein sequencing of an authentic elongation factor Tu from T. thermophilus HB8. Expression of this tuf gene in E.coli minicells provided a low amount of immuno-precipitable thermophilic EF-Tu. Affinity labeling of the T. thermophilus EF-Tu and sequence comparison with homologous proteins from other organisms were ussd to identify the gusnosine-nucleotide binding domain.Keywords
This publication has 28 references indexed in Scilit:
- The primary structure of the α subunit of human elongation factor 1European Journal of Biochemistry, 1986
- Structural homology between elongation factors EF - Tu from Bacillus stearothermophilus and Escherichia coli in the binding site for aminoacyl-tRNAEuropean Journal of Biochemistry, 1986
- The Amino Acid Sequence of the Tyrosy1-tRNA Synthetase from Bacillus stearothermophilusEuropean Journal of Biochemistry, 1983
- The nucleotide sequence of tufB and four nearby tRNA structural genes of Escherichia coliGene, 1980
- The nucleotide sequence of the cloned tufA gene of Escherichia coliGene, 1980
- Thermal stability and protein structureBiochemistry, 1979
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Protein expression in E. coli minicells by recombinant plasmidsCell, 1977
- Bacteriophage Qβ Replicase Contains the Protein Biosynthesis Elongation Factors EF Tu and EF TsProceedings of the National Academy of Sciences, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970