Modulation of pyruvate dehydrogenase kinase activity in cultured hepatocytes by glucagon and n-octanoate
- 15 February 1986
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 234 (1) , 233-236
- https://doi.org/10.1042/bj2340233
Abstract
The activity of pyruvate dehydrogenase kinase in extracts of mitochondria from rat hepatocytes cultured for 21 h in medium 199 was increased 2.5-fold by the presence of 55 nM-glucagon and 1 mM-sodium n-octanoate in the culture medium. The change was comparable with that induced in vivo by 48 h starvation. The potential contribution of branched-chain complex to estimates of PDH-complex activity in rat liver mitochondria has been defined.This publication has 18 references indexed in Scilit:
- Characterization of the effects of Ca2+ on the intramitochondrial Ca2+-sensitive enzymes from rat liver and within intact rat liver mitochondriaBiochemical Journal, 1985
- Effects of branched chain alpha-ketoacids on the metabolism of isolated rat liver cells. III. Interactions with pyruvate dehydrogenase.Journal of Biological Chemistry, 1980
- The mode of regulation of pyruvate dehydrogenase of lactating rat mammary gland. Effects of starvation and insulinBiochemical Journal, 1978
- Enhanced activity of pyruvate dehydrogenase kinase in rat heart mitochondria in alloxan‐diabetes or starvationFEBS Letters, 1978
- Conversion of inactive (phosphorylated) pyruvate dehydrogenase complex into active complex by the phosphate reaction in heart mitochondria is inhibited by alloxan-diabetes or starvation in the ratBiochemical Journal, 1978
- Branched chain 2‐oxo‐acid dehydrogenase complex of rat liverFEBS Letters, 1978
- Relationshiop between phosphorylation and activity of pyruvate dehydrogenase in rat liver mitochondria and the absence of such a relationship for pyruvate carboxylase.Journal of Biological Chemistry, 1978
- Calcium metabolism in rat hepatocytesBiochemical Journal, 1978
- Diabetes and the control of pyruvate dehydrogenase in rat heart mitochondria by concentration ratios of adenosine triphosphate/adenosine diphosphate, of reduced/oxidized nicotinamide-adenine dinucleotide and of acetyl-coenzyme A/coenzyme A.Biochemical Journal, 1977
- α-KETO ACID DEHYDROGENASE COMPLEXES, X. REGULATION OF THE ACTIVITY OF THE PYRUVATE DEHYDROGENASE COMPLEX FROM BEEF KIDNEY MITOCHONDRIA BY PHOSPHORYLATION AND DEPHOSPHORYLATIONProceedings of the National Academy of Sciences, 1969