INTERACTION OF APOA-II FROM HUMAN HIGH-DENSITY LIPOPROTEIN WITH LYSOLECITHIN

  • 1 January 1977
    • journal article
    • research article
    • Vol. 252  (11) , 3684-3688
Abstract
The effects of lysolecithin and hexadecyltrimethylammonium bromide on the structure and stability of apoA-II from human high density lipoprotein were evaluated by circular dichroism and fluorescence measurements. There is a profound enhancement in the stability of apoA-II to guanidinium hydrochloride denaturation when it forms a phospholipid complex with lysolecithin micelles. This complex is not only resistant to guanidinium hydrochloride denaturation, but it can be formed in a 6 M solution of this denaturant. The behavior of apoA-II in the native human high density protein is much closer to that of the lysolecithin apoA-II complex than to that of the free apoA-II.