CELL KINETICS AND RADIOSENSITIVITY OF HUMAN LYMPHOCYTES STIMULATED BY PHYTOHEMAGGLUTININ,WISTARIA FLORIBUNDAORLENTIL LECTIN
- 1 December 1982
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Genetics and Cytology
- Vol. 24 (6) , 761-769
- https://doi.org/10.1139/g82-081
Abstract
The cell kinetics and the radiosensitivities of human lymphocytes (4 donors) exposed to 200 rad of X-rays and stimulated with phytohemagglutinin (PHA), W. floribunda (WFA) or L. culinaris (LcH-A) extracts were compared after cultivation times from 42 up to 54 h. All these mitogens are considered activating primarily T lymphocytes. PHA is a tetrameric molecule, whereas WFA and LcH-A are dimeric structures having only 2 reactive sites. PHA displays a higher mitogenic activity than WFA, while LcH-A is much less active than PHA and WFA. After 42 h of culturing, only metaphases of the 1st mitosis are found, irrespective of the mitogen used. With increasing cultivation times, differences in the cell kinetics have been observed for the different mitogens. No differences in radiosensitivity of lymphocytes stimulated by these mitogens were observed when cells are analyzed exclusively in their 1st mitosis.This publication has 23 references indexed in Scilit:
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