Silicon Nanoparticles as a Luminescent Label to DNA
- 28 February 2004
- journal article
- Published by American Chemical Society (ACS) in Bioconjugate Chemistry
- Vol. 15 (2) , 409-412
- https://doi.org/10.1021/bc030047k
Abstract
We successfully conjugated 1-2 nm diameter silicon nanoparticles to a 5'-amino-modified oligonucleotide (60mer) that contains a C6 linker between amide and phosphate groups. The conjugation was implemented via two photoinduced reactions followed by a DNA labeling step through formation of a carboxamide bond. Photoluminescence of the conjugates is dominated by two blue bands (400 and 450 nm maximal) under 340 nm excitation. The quantum yield of oligonucleotide-conjugated nanoparticles was determined to be 0.08 as measured against quinine sulfate in 0.1 M HClO(4) as a reference standard. We report a conjugation process that allows labeling of Si nanoparticles to an oligonucleotide in aqueous solutions. Ways to further optimize the procedure in order to achieve narrower and brighter photoluminescence are discussed.Keywords
This publication has 8 references indexed in Scilit:
- Organometallic Chemistry on Silicon and Germanium SurfacesChemical Reviews, 2002
- Observation of a magic discrete family of ultrabright Si nanoparticlesApplied Physics Letters, 2002
- Oxide and hydrogen capped ultrasmall blue luminescent Si nanoparticlesApplied Physics Letters, 2000
- Controlled Functionalization and Multistep Chemical Manipulation of Covalently Modified Si(111) Surfaces1Journal of the American Chemical Society, 1999
- Bioreactive Self-Assembled Monolayers on Hydrogen-Passivated Si(111) as a New Class of Atomically Flat Substrates for Biological Scanning Probe MicroscopyJournal of Structural Biology, 1997
- Alkyl Monolayers on Silicon Prepared from 1-Alkenes and Hydrogen-Terminated SiliconJournal of the American Chemical Society, 1995
- NEW PHOTOLABELING AND CROSSLINKING METHODSAnnual Review of Biochemistry, 1993
- 4'-(1-Azi-2,2,2-trifluoroethyl)phenylalanine, a photolabile carbene-generating analog of phenylalanineJournal of the American Chemical Society, 1984