Isolation and characterization of cDNA clones specifically expressed in testicular germ cells

Abstract
We have cloned cDNAs involved in germ cell‐specific expression. For this, a subtracted cDNA library was generated by subtracting cDNAs derived from supporting cells of mutant testis from wild‐type testis cDNAs. Detailed analyses of mRNA expression revealed that the genes corresponding to the cloned cDNAs were exclusively expressed in testes and were developmentally controlled.