Abstract
Protein kinases (3) were distinguished in T. gambiense extract. The enzymes preferred phosvitin, histone and protamine as acceptor proteins, respectively. The amino acid residues of the acceptor proteins which were phosphorylated by these protein-kinase activities were serine, and to lesser extent, threonine. The protein kinase activities were neither affected by cyclic nucleotides nor by cyclic AMP receptors. The MW of these protein kinases were > 200,000, 95,000 and 37,000, respectively. The activities of all 3 protein kinases were affected to varying degrees by nucleotides and nucleosides.