RESOLUTION OF THE APOENZYME AND COENZYME OFL-GLUTAMIC ACID DECARBOXYLASE FROM SUNFLOWER COTYLEDONS
- 1 June 1961
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry and Physiology
- Vol. 39 (6) , 1055-1059
- https://doi.org/10.1139/o61-106
Abstract
Methods for the resolution of glutamic acid decarboxylase of sunflower cotyledons into the apo- and co-enzyme moieties are described. The apoenzyme can be separated from codecarboxylase by repeated acid – ammonium sulphate treatment with about 90% resolution and 40–50% inactivation. A codecarboxylase factor can be isolated as the barium salt following denaturation and precipitation of the protein by trichloracetic acid.This publication has 9 references indexed in Scilit:
- ANREICHERUNG UND EIGENSCHAFTEN EINER TRANSAMINASE AUS BIERHEFE1958
- THE PROSTHETIC GROUP OF PHOSPHORYLASEProceedings of the National Academy of Sciences, 1957
- The isolation of pyridoxal-5-phosphate from crystalline muscle phosphorylaseBiochimica et Biophysica Acta, 1957
- Glutamic Acid Decarboxylase of Squash and RadishBotanical Gazette, 1954
- GLUTAMIC CARBOXYLASE OF THE MATURE WHEAT LEAFCanadian Journal of Botany, 1952
- An l-glutamic acid decarboxylase from barleyBiochemical Journal, 1951
- DETERMINATION OF SERUM PROTEINS BY MEANS OF THE BIURET REACTIONJournal of Biological Chemistry, 1949
- Studies on bacterial amino-acid decarboxylasesBiochemical Journal, 1943