Retinola induced changes in cAMP‐dependent protein kinase activity detected by a new minigel assay
- 12 February 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 261 (1) , 81-84
- https://doi.org/10.1016/0014-5793(90)80641-u
Abstract
A new method for the resolution of protein kinases in nondenaturing minigels was used to analyze the type of PKA induced by retinyl acetate in transformed mouse 10T cells. Protein kinases in 0.5 μl aliquots of cell extracts were resolved on nondenaturing gradient Phastgels and assayed for PKA activity in situ using a specific peptide substrate and [32P]ATP. Retinyl acetate caused a decrease in type I and an increase in type II PKA isoforms after about 36 h in culture. The system provides a quick and sensitive means for analyzing the activity of PKA isoforms from small numbers of cells.Keywords
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