ENZYMIC OXIDATION AND REDUCTION OF CORTISOL WITH BACILLUS CEREUS
- 1 January 1976
- journal article
- research article
- Published by Microbiology Research Foundation in The Journal of General and Applied Microbiology
- Vol. 22 (1) , 25-33
- https://doi.org/10.2323/jgam.22.25
Abstract
The enzymatic transformation of cortisol with B. cereus was examined in the presence of some redox agents and enzyme inhibitors. At the early phases of fermentation, the enzymic 1,2-dehydrogenation reaction of cortisol was highly accelerated in the presence of oxidized glutathione. Thereafter, the enzymic reduction of the 20-keto group of cortisol and prednisolone was noticeably enhanced. In the presence of reduced glutathione, prednisolone was quantitatively converted to the 20.beta.-hydroxy derivative. The bioconversion of cortisol to prednisolone, 20.beta.-hydroxycortisol and 20.beta.-hydroxyprednisolone was also activated in the presence of azide and cyanide. With iodoacetate, the 1,2-dehydrogenation reaction was promoted while the 20-keto reduction was markedly repressed. Pretreatment of the bacterial cells with various C19 and C21 steroids and with cholesterol affected the transformation pattern in different manners. The 1,2-dehydrogenation reaction of cortisol was considerably induced when the cells were pretreated with .DELTA.1-dehydroprogesterone, .DELTA.1,4-androstadienedione and .DELTA.1-dehydrotestosterone.Keywords
This publication has 2 references indexed in Scilit:
- Enzymic dehydrogenation of steroid A ringBiochimica et Biophysica Acta, 1959
- 20β-Hydroxy-steroid-dehydrogenase, ein neues kristallines enzymBiochimica et Biophysica Acta, 1959