Calcification. XII. Cation-Linked Inhibition by Fluoride and Cyanide Ions in -Glycerophosphate Medium.

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Abstract
The initial rate of calcification of rachitic rat cartilage in vitro is retarded by 10-4 [image] F ion to the same extent in beta-glycerophosphate as in inorganic phosphate media. With more prolonged incubation there is a relief of inhibition unless Mg is included in the calcifying solns. F, 10-3 [image], is similarly effective in preventing mineralization in beta-glycero-phosphate or inorganic phosphate, provided the soln. contains Mg. In the absence of Mg, as much or more calcification occurs in 10-3 [image] F soln. as in the F-free control. There is a comparable decrease in inhibition by Mg-F at higher levels of organic and inorganic phosphate. Cyanide ion has no effect on calcification unless Mg, Sr, Ba, or manganous ions are present, in which case an equivalent degree of inhibition is noted in beta-glycerophosphate and inorganic phosphate media. With increasing P concn. there is a relief of inhibition in both media. The striking similarity in response of the calcifying mechanism in both beta-glycero- phosphate and inorganic phosphate solns. to the action of F or cyanide ions would seem to preclude the existence of 2 wholly discrete mechanisms of calcification.

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