Cloning and characterization of the human gene for the α‐subunit of Gi2, a GTP‐binding signal transduction protein
- 23 May 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 232 (2) , 333-340
- https://doi.org/10.1016/0014-5793(88)80764-6
Abstract
We isolated and characterized human genomic clones encompassing the gene for the α-subunit of Gi2, a GTP-binding signal transduction protein abundantly expressed in myeloid cells. The gene is divided into 9 exons and spans 23.5 kb. Exons 2, 6 and 7 encode putative guanine nucleotide-binding domains that are highly conserved among GTP-binding proteins. A polyadenylation signal located within exon 9 predicts an MRNA size (∼ 2.3 kb) several hundred bases longer than that of published cDNAs, and consistent with the size seen on RNA blot hybridization. Primer extension and S1 nuclease analysis determined a major and several minor transcriptional start sites. The first exon and 5′ flanking region are highly G+ C rich, contain several GC boxes (SP1 transcription factor binding sites), a CAAT box, and lack a TATA box. The presumptive promoter region is thus similar to that of ras and other widely expressed genesKeywords
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