Chemical deglycosylation on a micro‐scale of membrane glycoproteins with retention of phosphoryl‐protein linkages
- 1 November 1989
- journal article
- research article
- Published by Wiley in Journal of Neuroscience Research
- Vol. 24 (3) , 398-401
- https://doi.org/10.1002/jnr.490240309
Abstract
We provide here an easy and convenient procedure for the chemical deglycosylation of membrane glycoproteins that can be done rapidly and inexpensively on large numbers of membrane samples on a microscale without prior isolation of the glycoprotein in question. We have used the procedure successfully on three different glycoproteins and additionally have shown that covalent linkages of the polypeptide to phosphoryl residues are preserved.Keywords
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