Basic Fibroblast Growth Factor Reduces the Gut and Liver Morphologic and Functional Injuries after Ischemia and Reperfusion

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Abstract
To explore the possible effects of basic fibroblast growth factor (bFGF) on ischemic gut and liver injuries after trauma. Animal models of superior mesenteric artery occlusion (45 minutes) and reperfusion (3 days) were used in this study. Seventy-two Wistar rats were divided into three groups of 24 rats each. The animals in bFGF-treated group were injected with 4 microg bFGF/rat in 0.15 mL normal saline solution containing heparin 0.1% (w/v) through the jugular vein at the onset of reperfusion. In the normal saline control group, all rats received the same vehicle, but without bFGF. Group 3 (sham-operated) underwent the same laparotomy procedure, but without superior mesenteric artery occlusion. Liver function parameters, the levels of serum tumor necrosis factor a, nitric oxide, superoxide dismutase, malondialdehyde (MDA), tissue bacterial examination, and pathologic study were used to evaluate the results. In bFGF-treated rats, the amounts of serum alanine transaminase and aspartate aminotransferase and serum tumor necrosis factor-alpha were reduced significantly at 6, 24, and 48 hours when compared with normal saline-treated rats. However, the changes in nitric oxide, superoxide dismutase, and MDA varied from each other as a function of time after injury. The amounts of nitric oxide were increased significantly at 6 hours in intestine in normal saline-treated rats and in liver in bFGF-treated rats (p Intravenous administration of bFGF may benefit in reducing gut and liver injuries after ischemia and reperfusion. The mechanisms of those effects may involve mitogenic and nonmitogenic effects of bFGF.