HEMOGLOBIN ESTIMATION BY THE REVOLUTIONARY COLORIMETRIC METHOD OF KENNEDY

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Abstract
In 1926 Robert P. Kennedy1published a method of colorimetry, for hemoglobin especially, that was so new, so valuable, that it is indeed regrettable that the very scholarliness of his paper seems to have concealed his discovery from most of us. With the exception of spectrophotometry, practically all previous colorimetry had comprised sundry expedients for the matching of colors. In Kennedy's hemoglobin method, by means of a light filter transmitting such a narrow band of wavelengths in the green as to be virtually monochromatic, he simply eliminated color from both standard and unknown, then matched light intensities instead—a totally different concept.2He could do this—and this is the whole point—because, as seen through his filter, red and gray both appear green. Not merely green, but when illuminated to equal brightness both appear of the identical hue of green. I have stated before8the basic principle as applied

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